Confocal microscopy service

The confocal microscope allows high-resolution imaging of fluorescent samples, making it a valuable tool in biological, biomedical and other scientific research.

Implementing

Experiments can be performed with established time lapses, with FRAP techniques, FRET, 3D reconstructions, Z-stack, colocalization analysis and various analyses of the resulting images. The service is available to all users, institutes and laboratories in the region, both public and private.

Methodology

The confocal microscope is distinguished by its ability to remove scattered light outside the focal plane of the sample. This is achieved by using a pinhole or disc opening system to block out scattered light, resulting in a sharper image and higher contrast.

Frequently asked questions

What are the technical characteristics of the equipment?
- Model: Confocal microscope Nikon C1 SiR Eclipse TiU. Nikon software EZ-C1 3.90.
- Fluor Plan 10x/0,30 DEC L/N1 /0,17 WD 1,0; Apo Plan 20x/0,75 DEC N2 /0,17 WD 1,0; Fluor 40x/1,30 OIL DIC H/N2 /0,17 WD 0,22; Apo Plan VC 60x A/1,20 WI /0,15,18,0,0,18 WD; Apo Plan N0,17/2/N2/NX
- Excitation laser lines: 408 nm, 488 nm and 561,4 nm.
- 5 detection filters: 450/40 nm (blue), 515/30 nm (green), 605/75 nm (red), 485/30 nm (cyan) and 550/50 nm (yellow).

What samples can be seen in the confocal microscope?
The confocal microscope is able to observe a wide variety of biological samples and materials containing autofluorescent or fluorophore-labeled cells and molecules. Some examples include: living cells and biological tissues (individual cells can be seen, cell cultures, biological tissues such as histological sections or living tissues in model organisms), subcellular structures, multicellular tissues and live samples in real time.

Is it necessary to make an appointment to use the service?
Yes, if you required to use the confocal microscope, request an appointment through the National Microscopy System: http://turnos.microscopia.mincyt.gob.ar/

Technical Responsible

Dr. Ignacio Cerrudo
icerrudo@agro.uba.ar