Phenotypic and cellular analysis of stomatal physiology in plants

The service includes the cultivation of plants of different species, preparation of isolated tissues, treatment of isolated plants and tissues, digitization and quantification of light field optical microscopy and fluorescence.
Light field microscopy, phase contrast and epifluorescence are performed. With respect to the phenotype it is offered to quantify the stomatal pattern, evaluate the dry and fresh biomass, relative water content, drying and quantification of the temperature of the foliar area, thermal infrared thermography (TIR) as an estimate of the stomatic behavior.

Methodology

Cultivation of model or agronomically interesting species under controlled conditions of light, humidity and temperature. The seeds are sterilized and placed in the optimal substrate for each species, then grown in controlled culture rooms. For the stomatal behavior in isolated tissues, cuttings of isolated epidermis are extracted, preincubated in solutions that favor opening and the promotion of opening or induction of closing is evaluated by optical microscopy. For estimation of stomatal behaviour in whole leaf, TIR images are taken of the different water conditions.

Frequently asked questions

How long does the service take?
Service delay depends on the species used to assess the phenotype and number of techniques required.

What kind of stimuli can I evaluate?
Any physical stimulus (light, temperature, etc.) or chemical (drugs, bioproducts) that may affect stomatal physiology.

Technical Responsible

Dr. Carlos García-Mata
2235950482
camata@mdp.edu.ar