Analysis of polysaccharides and reducing sugars in biological samples

We perform quantitative analysis of polysaccharides and reducing sugars in biological samples. We use spectrophotometric techniques to determine the amount of polysaccharides such as chitosans, oligoquitosans and starch, as well as the concentration of reducing sugars in liquid samples provided by the customer.

Methodology

The service consists of the quantitative determination of polysaccharides (chitosans, oligoquitosans, starch) or reducing sugars using spectrophotometric techniques. For the determination of starch, the potassium iodine-iodide chemical method described by Sensabaugh Jr. & Rush (1972) will be used. For the determination of chitosans it will be used the methodology based on depolymerization with sodium nitrite followed by the thiobarbituric acid reaction described by Badawy (2012). Oligo-chitosans will be quantified by the MBTH method (3-methyl-2-benzothiazolone hydrazine) according to Horn & Eijsink (2004). The determination of reducing sugars will be made by the Somogyi-Nelson Ashwell method (1957). In addition to the methodologies described, other spectrophotometric methods described in literature may be used, as required by the sample to be analyzed.

Implementing

Our service has several applications in scientific research, the food, pharmaceutical, cosmetic and biomedical industry.

Technical Responsible

Dr. Andrea Yamila Mansilla
2235186382
amansill@mdp.edu.ar