Analysis of relative gene expression using real-time PCR

Analysis of relative gene expression using real-time PCR
Real-time PCR (qPCR) is a methodology that allows the quantification of DNA and RNA with a high sensitivity, being the most chosen method at present. The services we offer include:
1) Extraction of nucleic acids by the TRIzol method.
2) Quantification by spectrophotometry (Nanodrop) and quality determination by electrophoresis in agarose gels.
3) Synthesis of cDNA (in the case of transcript analysis).
4) Advice on the design or design of primers and validation of the test (efficiency, specificity and dynamic range).
5) Real-time PCR reaction preparation, programming and run for gene expression determination using the SYBR Green method using the 96-well StepOne Plus (Applied Biosystem) equipment.
6) Analysis of results by relative expression of the gene of interest in control samples and treated according to ΔΔCt methods (Livak and Schmittgen, 2001) or N0 (Ruijter et al., 2009).

Implementing

This service allows the relative quantification of gene expression between samples of a condition/treated compared to control samples. The determination of gene expression is important for both basic and applied science, for example in areas of industry and health.

Frequently asked questions

Is it necessary to contract all services together?
No, services can be purchased individually.

Can I evaluate gene expression using primers that I have designed and available?
Yes, the user can deliver an aliquot of their own primers to the service.

Can nucleic acids be extracted from any sample through the service offered?
No, we have biosecurity level 1, so we can’t extract nucleic acids from pathogenic or human samples. However, we can analyze the expression of genes of these samples if the user provides the service of the cDNA of them.

Technical Responsible

Dr. María Belén Fernández
2235826835
mbfernan@mdp.edu.ar