Freeze-drying of biological products

Lyophilization is the process by which a frozen sample is dehydrated by vacuum action, without going through the liquid phase. This service includes the lyophilization of biological products of biosecurity level I-II, being able to be organic and inorganic compounds.

Methodology

Samples are received in format to be agreed with the service (test tubes, plates, microtubes, etc.) and the same elements with lyophilized content are delivered.

Implementing

Biological samples (proteins, antibodies, genetic material, animal and plant tissues, among others) and chemical samples (extracts of specific chemical compounds, pharmaceutical products, standards for other uses) requiring dehydration for longer shelf life during storage or transport.

Frequently asked questions

How is the freeze drying process?
The equipment is pre-cooled at -52 ºC. The containers with the samples are covered with parafilm (with small holes). Freeze-dried samples are frozen at -80ºC in an ultrafreezer for at least 3h before beginning the freeze-dried cycle. The frozen samples are then placed in the freeze-dried trays and the vacuum is generated in the equipment. Temperature and vacuum are monitored during the freeze drying cycle. After the freeze-drying cycle is completed, the samples are stored according to the user’s need.

How do I send the sample for lyophilization?
Depending on the characteristics of the sample, it can be received at room temperature and we take care of freezing it in the laboratory, refrigerated or in liquid nitrogen.

How long will it take to freeze my sample?
It depends on several factors, including the type of sample to be lyophilized, the volume of the sample and the size of the container containing the sample. Most samples freeze-dried between 24 to 48 hours. The larger the volume, the smaller the contact surface, the longer the freeze drying cycle takes. A sample with a low freezing point will also need a longer cycle.

What are the advantages of freeze drying a sample compared to other drying-concentrate methods?
Products are obtained that can be regenerated very easily while maintaining their original physical, chemical, biological and organoleptic properties. It is an ideal process for drying thermolabile or oxidation-susceptible substances. The final moisture content is extremely low.

Technical Responsibles

Dr. Lucía Zalazar
zalazar@mdp.edu.ar

Dr. Juan Pablo Córdoba
2235183324
jpcordoba@mdp.edu.ar